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alexa fluor 488 affinipure donkey anti mouse igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 488 affinipure donkey anti mouse igg

    Alexa Fluor 488 Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alexa+488/Alexa+Fluor+488+AffiniPure+Donkey+Anti-Mouse+IgG/pmc13657110-418-13-23
    Average 96 stars, based on 1825 article reviews
    alexa fluor 488 affinipure donkey anti mouse igg - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models"

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    Journal: iScience

    doi: 10.1016/j.isci.2026.117673


    Figure Legend Snippet:

    Techniques Used: Recombinant, Mutagenesis, Software, Analysis, Silicon

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Orbitofrontal cortex drives predictive filtering of sensory responses.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibody: rabbit anti-NMDAR1 (Abcam, ab52177, lot 1030057-4) Secondary antibody: donkey anti-rabbit IgG, Alexa 488 (Jackson ImmunoResearch, 711-545-152, lot 134352) Validation The antibodies were confirmed in literatures (DOI: 10.1016/j.bbr.2020.112659; DOI: 10.3389/fpsyt.2020.00515) and were previously tested in wild-type animals in the lab. ..

    Flow Cytometry:

    Article Title: Orbitofrontal cortex drives predictive filtering of sensory responses.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibody: rabbit anti-NMDAR1 (Abcam, ab52177, lot 1030057-4) Secondary antibody: donkey anti-rabbit IgG, Alexa 488 (Jackson ImmunoResearch, 711-545-152, lot 134352) Validation The antibodies were confirmed in literatures (DOI: 10.1016/j.bbr.2020.112659; DOI: 10.3389/fpsyt.2020.00515) and were previously tested in wild-type animals in the lab. ..

    Magnetic Resonance Imaging:

    Article Title: Orbitofrontal cortex drives predictive filtering of sensory responses.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibody: rabbit anti-NMDAR1 (Abcam, ab52177, lot 1030057-4) Secondary antibody: donkey anti-rabbit IgG, Alexa 488 (Jackson ImmunoResearch, 711-545-152, lot 134352) Validation The antibodies were confirmed in literatures (DOI: 10.1016/j.bbr.2020.112659; DOI: 10.3389/fpsyt.2020.00515) and were previously tested in wild-type animals in the lab. ..

    Biomarker Discovery:

    Article Title: Orbitofrontal cortex drives predictive filtering of sensory responses.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibody: rabbit anti-NMDAR1 (Abcam, ab52177, lot 1030057-4) Secondary antibody: donkey anti-rabbit IgG, Alexa 488 (Jackson ImmunoResearch, 711-545-152, lot 134352) Validation The antibodies were confirmed in literatures (DOI: 10.1016/j.bbr.2020.112659; DOI: 10.3389/fpsyt.2020.00515) and were previously tested in wild-type animals in the lab. ..

    Immunohistofluorescence:

    Article Title: Anti-tau VHH therapy against PHF6: a safe approach to slowing the phenotype of tau pathology.
    Article Snippet: .. Immunohistofluorescence For extraVHH, brain sections were saturated in normal goat serum (1/100; S-1000, Vector), then incubated with the primary antibody anti-Fc conjugated with Alexa 488 (AffiniPure Goat Anti-mouse IgG, Fcγ fragment specific; 1/500; 115–545-008, Jackson ImmunoResearch) for 1 h at room temperature in PBS-0.2% Triton X-100. .. Then they were incubated with the antibody anti-tau AT8 biotinylated (1/500; MN1020B, Invitrogen) ON at 4 °C in PBS-0.2% Triton X-100.

    Incubation:

    Article Title: Anti-tau VHH therapy against PHF6: a safe approach to slowing the phenotype of tau pathology.
    Article Snippet: .. Immunohistofluorescence For extraVHH, brain sections were saturated in normal goat serum (1/100; S-1000, Vector), then incubated with the primary antibody anti-Fc conjugated with Alexa 488 (AffiniPure Goat Anti-mouse IgG, Fcγ fragment specific; 1/500; 115–545-008, Jackson ImmunoResearch) for 1 h at room temperature in PBS-0.2% Triton X-100. .. Then they were incubated with the antibody anti-tau AT8 biotinylated (1/500; MN1020B, Invitrogen) ON at 4 °C in PBS-0.2% Triton X-100.

    Article Title: PEG10-ORF1 programs trophoblast progenitor development for placental labyrinth formation
    Article Snippet: The primary antibodies used were: rat monoclonal anti-CD31 (1:100; BD Biosciences, 550274); rabbit polyclonal anti-PEG10-ORF1 (1:100; Cell Signaling Technology, #93531); rat monoclonal anti-CD326 (1:100; BD Biosciences, 552370); rabbit polyclonal anti-Ki67 (1:100; Abcam, ab15580); rabbit polyclonal anti-Phospho-Histone H3 (Ser10) (1:200; Cell Signaling Technology, #9701); rabbit monoclonal anti-cleaved caspase3 (1:400; Cell Signaling Technology, #9664); rat monoclonal anti-CD71 (1:100; SantaCruz, sc-59112). .. After three washes with PBS (5 min each), sections were incubated with secondary antibodies in blocking buffer at 4 °C overnight: donkey anti-rat IgG conjugated with Alexa 488 (1:500; Jackson ImmunoResearch, 712-545-153); donkey anti-rabbit IgG conjugated with Cy3 (1:500; Jackson ImmunoResearch, 711-165-152). .. Sections were washed again in PBS (3 × 5 min) and mounted with VECTASHIELD-Hardset (Vector Laboratories) containing 1 μg/ml DAPI.

    Article Title: SOX11-mediated CBLN2 Upregulation Contributes to Neuropathic Pain through NF-κB-Driven Neuroinflammation in Dorsal Root Ganglia of Mice.
    Article Snippet: Sections were blocked with 1% BSA and incubated with the following primary antibodies: CBLN2 (Rabbit, 1:500; Invitrogen, PA5-101514, USA), neuronal class III β-tubulin (TUJ1; Mouse 1:1000; Millipore MAB1637, Germany), glutamine synthetase (GS; Mouse 1:500; Millipore MAB302, Germany), ionized calcium binding adapter molecule 1 (IBA-1; Goat, 1:500; Abcam ab5076, UK), 200 kDa neurofilament protein (NF200; Mouse, 1:1000; Millipore, MAB5266, Germany), calcitonin gene-related peptide (CGRP; Goat, 1:3000; Bio-Rad 1720- 9007, USA), isolectin B4 (IB4; 1:50; Sigma L2895, USA), NF-κB p65 (Rabbit, 1:1000; CST, 8242S, USA), PhosphoNF-κB p65 (Rabbit, 1:1000; CST, 3033S, USA) and SOX11 (Rabbit, 1:50; MCE HY-P80332, USA). .. The sections were then incubated for 2 h at room temperature with Cy3‐ or Alexa 488‐conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, 715-167-003, 715-547-003, and 106- 546-003, USA). .. Fluorescence images were acquired using a Leica SP8 confocal microscope (Germany).

    Article Title: Anti-tau VHH therapy against PHF6: a safe approach to slowing the phenotype of tau pathology
    Article Snippet: .. For extraVHH, brain sections were saturated in normal goat serum (1/100; S-1000, Vector), then incubated with the primary antibody anti-Fc conjugated with Alexa 488 (AffiniPure Goat Anti-mouse IgG, Fcγ fragment specific; 1/500; 115–545-008, Jackson ImmunoResearch) for 1 h at room temperature in PBS-0.2% Triton X-100. .. Then they were incubated with the antibody anti-tau AT8 biotinylated (1/500; MN1020B, Invitrogen) ON at 4 °C in PBS-0.2% Triton X-100.

    Article Title: SOX11-mediated CBLN2 Upregulation Contributes to Neuropathic Pain through NF-κB-Driven Neuroinflammation in Dorsal Root Ganglia of Mice
    Article Snippet: Sections were blocked with 1% BSA and incubated with the following primary antibodies: CBLN2 (Rabbit, 1:500; Invitrogen, PA5-101514, USA), neuronal class III β-tubulin (TUJ1; Mouse 1:1000; Millipore MAB1637, Germany), glutamine synthetase (GS; Mouse 1:500; Millipore MAB302, Germany), ionized calcium binding adapter molecule 1 (IBA-1; Goat, 1:500; Abcam ab5076, UK), 200 kDa neurofilament protein (NF200; Mouse, 1:1000; Millipore, MAB5266, Germany), calcitonin gene-related peptide (CGRP; Goat, 1:3000; Bio-Rad 1720-9007, USA), isolectin B4 (IB4; 1:50; Sigma L2895, USA), NF-κB p65 (Rabbit, 1:1000; CST, 8242S, USA), Phospho-NF-κB p65 (Rabbit, 1:1000; CST, 3033S, USA) and SOX11 (Rabbit, 1:50; MCE HY- P80332 , USA). .. The sections were then incubated for 2 h at room temperature with Cy3‐ or Alexa 488‐conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, 715-167-003, 715-547-003, and 106-546-003, USA). .. Fluorescence images were acquired using a Leica SP8 confocal microscope (Germany).

    Article Title: Characterization of the N-terminal region of Crtac1B/LOTUS, an endogenous Nogo receptor-1 antagonist, required for secretion and soluble function.
    Article Snippet: To confirm protein overexpression in the binding assay, cultured cells were incubated with primary antibodies (antiNgR1: catalog #AF1440, 0.8 μg/ml, RRID: AB_2183731 R&D; antip75: catalog #AB8874, 1:1000, RRID: AB_306827, Abcam, Cambridge, UK; antiGM130: 0.5 μg/ml, catalog #610822, Becton and Dickinson, NJ, USA) at 37 ◦C, 5 % CO2 for 1 h, followed by fixation in 4 % paraformaldehyde in PBS for 1 h at room temperature (RT). .. After washing with tris buffer saline (0.1 % Tween 20 (TBST)) three times, cells were incubated with Alexa 488-conjugated secondary antibodies (antiNgR1: 0.75 μg/ml, catalog #705-545-003, RRID: AB_2340046; antip75, 0.75 μg/ml, catalog #111-545-003, RRID: AB_2338046, both Jackson ImmunoResearch Laboratories, PA, USA) for 1 h at RT. ..

    Blocking Assay:

    Article Title: PEG10-ORF1 programs trophoblast progenitor development for placental labyrinth formation
    Article Snippet: The primary antibodies used were: rat monoclonal anti-CD31 (1:100; BD Biosciences, 550274); rabbit polyclonal anti-PEG10-ORF1 (1:100; Cell Signaling Technology, #93531); rat monoclonal anti-CD326 (1:100; BD Biosciences, 552370); rabbit polyclonal anti-Ki67 (1:100; Abcam, ab15580); rabbit polyclonal anti-Phospho-Histone H3 (Ser10) (1:200; Cell Signaling Technology, #9701); rabbit monoclonal anti-cleaved caspase3 (1:400; Cell Signaling Technology, #9664); rat monoclonal anti-CD71 (1:100; SantaCruz, sc-59112). .. After three washes with PBS (5 min each), sections were incubated with secondary antibodies in blocking buffer at 4 °C overnight: donkey anti-rat IgG conjugated with Alexa 488 (1:500; Jackson ImmunoResearch, 712-545-153); donkey anti-rabbit IgG conjugated with Cy3 (1:500; Jackson ImmunoResearch, 711-165-152). .. Sections were washed again in PBS (3 × 5 min) and mounted with VECTASHIELD-Hardset (Vector Laboratories) containing 1 μg/ml DAPI.

    Saline:

    Article Title: Characterization of the N-terminal region of Crtac1B/LOTUS, an endogenous Nogo receptor-1 antagonist, required for secretion and soluble function.
    Article Snippet: To confirm protein overexpression in the binding assay, cultured cells were incubated with primary antibodies (antiNgR1: catalog #AF1440, 0.8 μg/ml, RRID: AB_2183731 R&D; antip75: catalog #AB8874, 1:1000, RRID: AB_306827, Abcam, Cambridge, UK; antiGM130: 0.5 μg/ml, catalog #610822, Becton and Dickinson, NJ, USA) at 37 ◦C, 5 % CO2 for 1 h, followed by fixation in 4 % paraformaldehyde in PBS for 1 h at room temperature (RT). .. After washing with tris buffer saline (0.1 % Tween 20 (TBST)) three times, cells were incubated with Alexa 488-conjugated secondary antibodies (antiNgR1: 0.75 μg/ml, catalog #705-545-003, RRID: AB_2340046; antip75, 0.75 μg/ml, catalog #111-545-003, RRID: AB_2338046, both Jackson ImmunoResearch Laboratories, PA, USA) for 1 h at RT. ..



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    Image Search Results


    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

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    Figure Lengend Snippet: IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Article Snippet: Cell proliferation was observed by laser confocal microscope at 24 h, 48 h, 72 h. Briefly, the proliferation of cells was assessed by BeyoClick EdU-488 Cell Proliferation Kit (Beyotime) according to the manufacturer’s instructions.

    Techniques: Migration, Control, Expressing, Analysis, EdU Assay, Fluorescence, Staining, Transwell Migration Assay, Transwell Assay

    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: Sections were then washed in 1 x PBS four times and incubated in Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L, Jackson ImmunoResearch, cat no: 715–545-150, West Grove, Pennsylvania), 1:200 dilution in 1% normal donkey serum in 0.5% Triton X-100 overnight at 4°C.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon